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Title

In-office bleaching effect with different concentrations of hydrogen peroxide on enamel color and demineralization

 

Authors

Bilal Ahmad Mir*, Muneesa Jan, Anu Sharma, Aamir Rashid Purra, Fayaz Ahmed Ahanger & Ajaz Amin Masoodi

 

Affiliation

Department of Conservative Dentistry and Endodontics, Government Dental College and Hospital, Srinagar, Jammu and Kashmir, India; *Corresponding author

 

Email

Bilal Ahmad Mir - E-mail: munesaandrabi@gmail.com

Muneesa Jan - E-mail: muneesaandrabi@gmail.com

Anu Sharma - E-mail: sharmanu999@gmail.com

Aamir Rashid Purra - E-mail: apurra76@gmail.com

Fayaz Ahmed Ahanger - E-mail: faahangar@yahoo.com

Ajaz Amin Masoodi - E-mail: ajazmsoodi@gmail.com

 

Article Type

Research Article

 

Date

Received April 1, 2026; Revised April 30, 2026; Accepted April 30, 2026, Published April 30, 2026

 

Abstract

Use of hydrogen peroxide in tooth bleaching can cause microscopic alterations such as increasedsurface depressions, irregularities and elevated surface roughness. Therefore, it is of interest to report the enamel surface effects of two in-office hydrogen peroxide (HP) bleaching gels. Forty-eight intact human premolars were sectioned mesio-distally, creating two halves, which were then assigned to 2 groups. Group I (35% HP with calcium; whiteness HP Blue 35%, FGM) or Group II (40% HP with fluoride/potassium nitrate; Opalescence Boost, Ultradent). Bleaching was performed in a single 40-minute session per manufacturer protocol. Baseline and post-treatment measurements were taken using a spectrophotometer (VITA Easyshade) for color and a laser fluorescence device (DIAGNOdent pen, KaVo) for quantifying demineralization. The 40% hydrogen peroxide gel with fluoride and potassium nitrate produced a superior whitening effect; both bleaching agents resulted in comparable levels of enamel surface demineralization.

 

Keywords

Colorimetry; DIAGNOdent; enamel demineralization; hydrogen peroxide(HP); split-tooth model; in vitro study, tooth bleaching

 

Citation

Mir et al. Bioinformation 22(4): 2395-2399 (2026)

 

Edited by

P Babaji

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.