Title
Authors
Affiliation
1Department of Pharmaceutical and Nutrition Sciences, Research Group, College of Pharmacy and Nutrition, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada; 2Department of Pediatrics and Adolescent Medicine, Division of Pediatric Endocrinology and Diabetes, Ulm University Medical Center Ulm, Ulm, Germany; *Corresponding author
Leilei Sun - E-mail: les206@mail.usask.ca
Martin Wabitsch - E-mail: Martin.Wabitsch@uniklinik-ulm.de
Jian Yang - E-mail: jian.yang@usask.ca
Meena Kishore Sakharkar - E-mail: meena.sakharkar@usask.ca
Article Type
Research Article
Date
Abstract
Adipogenesis involves adipocyte differentiation and synthesis and storage of fats. PPAR-δ is the master regulator of adipogenesis and regulates genes for adipocyte differentiation, lipogenesis, adipocyte survival and adipokine secretion. Central carbon metabolism (CCM) comprises of three key pathways glycolysis, tricarboxylic acid (TCA) cycle and pentose phosphate pathway (PPP). CCM utilizes carbon sources to provide energy and building blocks for lipogenesis. Several targets of transcription factor PPAR-δ have been identified in CCM pathways. However, the exact mechanism of PPAR-δ modulation in adipogenesis via CCM remains elusive. Therefore, it is of interest to evaluate the effects of PPAR-δ on CCM in differentiated human SGBS adipocytes by real time-qPCR and metabolomics using its agonist 15d-PGJ2 and antagonist GW9662. Surprisingly, our results suggest that PPAR-δ rather than PPAR-δ is likely to be the key modulator of CCM in differentiated adipocytes at least under current experimental conditions with both 15d PGJ2 and GW9662 being PPAR-δ agonists, although PPAR-δ plays a crucial role in adipogenesis and lipogenesis. This data provides insight to manage obesity and diabetes using PPAR-δ agonists. Further studies are warranted to explore the regulation of CCM by PPARs in adipocytes.
Keywords
PPAR-δ, PPAR-δ, adipogenesis, 15d-PGJ2, Central Carbon Metabolism (CCM)
Citation
Sun et al. Bioinformation 22(4): 2419-2429 (2026)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
