Title
Human mesenchymal stem cell differentiation on bioactive glass-coated implants
Authors
BS Vinay Dutta1,*, Omkar Shete2, Anuradha Venkataramani1, Anuradha Nemane2, Pankaj Prakash Kharade3 & TS Priyanka1
Affiliation
1Department of Prosthodontics, DA Pandu Memorial RV Dental College, Bengaluru, India; 2Department of Prosthodontics, GD Pol Dental College and Hospital PG Institute, Kharghar, Navi Mumbai, India; 3Department of Prosthodontics, Dr. ZA Dental College, AMU Aligarh, India; *Corresponding author
BS Vinay Dutta - E-mail: vinay.bks@gmail.com
Omkar Shete - E-mail: omkarrshete@gmail.com
Anuradha Venkataramani - E-mail: anurad.venka@gmail.com
Anuradha Nemane - E-mail: anuradhanemane@gmail.com
Pankaj Prakash Kharade - E-mail: drpankaj.pgi08@gmail.com
TS Priyanka - E-mail: preecool.ts@gmail.com
Article Type
Research Article
Date
Received April 1, 2026; Revised April 30, 2026; Accepted April 30, 2026, Published April 30, 2026
Abstract
Successful osseointegration of titanium implants remains a major challenge because the ideal bioactive surface coating and its specific influence on stem cell osteogenic differentiation are not yet fully understood. Therefore, it is of interest to evaluate the effect of bioactive glass (BG) coatings on the osteogenic differentiation of human bone marrow mesenchymal stem cells cultured on titanium implant discs. Forty-eight commercially pure titanium discs were divided into four groups (n = 12 each): uncoated control, 45S5 BG- coated, strontium-substituted BG-coated (Sr-BG) and zinc-substituted BG-coated (Zn-BG) and assessed at days 7, 14 and 21 for cell viability, alkaline phosphatase activity, osteocalcin expression, mineralized nodule formation and expression of RUNX2, COL1A1, OCN and OPN. All BG-coated groups demonstrated significantly greater cell viability, ALP activity and mineralized calcium deposition than the uncoated control (p < 0.05), while the Sr-BG group showed the highest RUNX2 and osteocalcin gene expression at day 21 (p < 0.001), followed by the Zn-BG group. Bioactive glass coating, particularly strontium-substituted BG, significantly enhanced osteogenic differentiation of hBMSCs on titanium surfaces and may improve the clinical success of osseointegration.
Keywords
Bioactive glass (BG), mesenchymal stem cells, osteogenic differentiation, titanium implants, surface coating, osseointegration
Citation
Dutta et al. Bioinformation 22(4): 2533-2538 (2026)
Edited by
Hiroj Bagde
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
