Title

Correlation between vitamin B12 levels and peripheral neuropathy in diabetic patients

Authors

MD Irfan^1,*, Poonam Motwani², Ritika Agrawal³, B Jayakrishnan⁴, Parth Jani⁵, Rosario Maristhanny García Ferrín⁶, Souvik Sur⁷ & Ram Rattan Negi⁸

Affiliation

¹Department of Physiology, Santosh Deemed to be University, Ghaziabad, Delhi NCR, India; ²Department of Physiology, Government Medical College (GMC), Ratlam, Madhya Pradesh, India; ³Department of Anaesthesiology, Gajra Raja Medical College, Gwalior, Madhya Pradesh, India; ⁴Department of Medicine, Educare Institute of Dental Sciences, Malappuram, Kerala, India; ⁵Department of General Medicine, Government Medical College, Bhavnagar, Gujarat, India; ⁶Department of Medicine, Universidad Central del Ecuador, Ecuador; ⁷Department of Chemistry, Teerthanker Mahaveer University, Moradabad, Uttar Pradesh, India; 8Department of Biochemistry, AIIMS, Bhopal, Madhya Pradesh, India; *Corresponding author

Email

MD Irfan - E-mail: mdirfankmc@gmail.com

Poonam Motwani - E-mail: poonammotwani17@gmail.com

Ritika Agrawal - E-mail: nameisritika1993@gmail.com

B Jayakrishnan - E-mail: jaykdr@gmail.com

Parth Jani - E-mail: parthjani13@gmail.com

Rosario Maristhanny García Ferrín - E-mail: rm.gf.92@outlook.com

Souvik Sur - E-mail: souvik31sur@gmail.com

Ram Rattan Negi - E-mail: ram.biochemistry@aiimsbhopal.edu.in

Article Type

Research Article

Date

Received April 1, 2026; Revised April 30, 2026; Accepted April 30, 2026, Published April 30, 2026

Abstract

Diabetic peripheral neuropathy is a common and debilitating complication of diabetes mellitus, significantly impacting the quality of life of affected individuals. Vitamin B12 is essential for nerve function and myelin synthesis and its deficiency can lead to neuropathy that resembles diabetic neuropathy. Long-term metformin use in diabetic patients increases the risk of vitamin B12 deficiency, making the relationship between the two critical to explore. The study evaluated the correlation between serum vitamin B12 levels and peripheral neuropathy in type 2 diabetes patients using clinical assessments and vitamin B12 measurement through a chemiluminescent immunoassay. Thus, we show the link between vitamin B12 deficiency and severity of peripheral neuropathy in type 2 diabetes patients on long-term metformin therapy.

Keywords

Vitamin B12 deficiency, diabetic peripheral neuropathy (DPN), type 2 diabetes mellitus, metformin therapy, neuropathy severity

Citation

Irfan et al. Bioinformation 22(4): 2624-2629 (2026)

Edited by

Ritik Kashwani

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.