Title

A comparative histochemical evaluation of tissue biomarkers in normal and pathological specimens: A retrospective study

Authors

Amit Kumar Srivastava¹^,*, Aishwarya Srivastava², Rohini Srivastava³, Prabha Verma⁴, Jyoti Batra⁵ & Yogesh Yadav⁶

Affiliation

¹Department of Anatomy, Government Medical College, Datia, Madhya Pradesh, India; ²Department of Biochemistry, Naraina Medical College and Research Centre, Kanpur, Uttar Pradesh, India; ³Department of Pathology, Naraina Medical College and Research Centre, Kanpur, Uttar Pradesh, India; ⁴Department of Biochemistry, GSVM Medical College, Kanpur, Uttar Pradesh, India; ⁵Department of Central Research Facility, Santosh Deemed to be University, Ghaziabad, Uttar Pradesh, India; ⁶Department of Anatomy, Saraswathi Institute of Medical Sciences, Hapur, Uttar Pradesh, India; *Corresponding author

Email

Amit Kumar Srivastava - E-mail: amitsrivastava48@gmail.com
Aishwarya Srivastava - E-mail: aishwaryasrivastava720@gmail.com
Rohini Srivastava - E-mail: drrohinisrivastava29@gmail.com
Prabha Verma - E-mail: drprabhaverma941@gmail.com
Jyoti Batra - E-mail: drjyotibatra89@gmail.com
Yogesh Yadav - E-mail: dryogeshanatomy@gmail.com

Article Type

Research Article

Date

Received May 1, 2026; Revised May 31, 2026; Accepted May 31, 2026, Published May 31, 2026
 

Abstract

Differences in histochemical biomarker expression between normal and pathological tissues remain inadequately characterized. Therefore, it is of interest to evaluate histochemical expression patterns of selected biomarkers in normal and pathological tissue samples. Hence, eighty archived specimens (40 normal and 40 pathological) were analyzed using histochemical staining and semi-quantitative scoring over two years. Pathological tissues showed higher staining intensity and altered distribution patterns, indicating changes in cellular metabolism and extracellular matrix composition. Thus, we show distinct biomarker expression patterns in diseased tissues, supporting their diagnostic and prognostic relevance.

Keywords

Histochemistry, tissue biomarkers, pathological specimens, normal tissue, retrospective study

Citation

Srivastava et al. Bioinformation 22(5): 3072-3076 (2026)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.