Title
Green synthesis and antioxidant evaluation of calcium oxide nanoparticles using Syzygium cumini fruit extract: An in vitro study
Authors
Srividhya Srinivasan1,*, K Saraswathi Gopal1, Shanmugam Rajesh Kumar2, B Anand3 & D. Thirumal Kumar4
Affiliation
1Department of Oral Medicine and Radiology, Faculty of Dentistry, Meenakshi Ammal Dental College and Hospital, Meenakshi Academy of Higher Education and Research, Chennai, Tamil Nadu, India; 2Nano biomedicine Lab, Centre for Global Health Research, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India; 3Department of Oral Medicine and Radiology, Ragas Dental College and Hospital, Chennai, Tamil Nadu, India; 4Central Research laboratory, Meenakshi Academy of Higher Education and Research, Chennai, Tamil Nadu, India; *Corresponding author
Srividhya Srinivasan - E-mail: drsrividhya.omrd@madch.edu.in; Phone: +91 9677047790
K. Saraswathi Gopal - E-mail: dr.saraswathik@gmail.com; Phone: +91 9443291115
S. Rajesh Kumar - E-mail: rajeshkumars.sdc@saveetha.com; Phone: +91 9629739263
B. Anand - E-mail: anandassasin@gmail.com; Phone: +91 9841698865
D. Thirumal Kumar - E-mail: thirumal@maher.ac.in; Phone: +91 8056295915
Article Type
Research Article
Date
Received June 1, 2026; Revised June 30, 2026; Accepted June 30, 2026, Published June 30, 2026
Abstract
The lack of safe, cost-effective, and eco-friendly antioxidant agents to combat oxidative stress–induced chronic diseases remains a significant challenge in biomedical research. Green synthesis offers a sustainable approach for producing bioactive nanoparticles using plant-derived phytochemicals. In this study, calcium oxide nanoparticles (CaO NPs) were synthesized using Syzygium cumin fruit extract. The formation of nanoparticles was confirmed by UV–Visible spectroscopy, while X-ray diffraction (XRD), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FTIR) revealed crystalline, spherical nanoparticles stabilized by phytochemical capping. The antioxidant activity of the synthesized CaO NPs was evaluated using DPPH, FRAP, ABTS, hydrogen peroxide, and nitric oxide assays at concentrations ranging from 10–50 µg/mL. The nanoparticles exhibited strong, concentration-dependent radical scavenging activity comparable to ascorbic acid. Data shows the potential of S. cumin-mediated CaO NPs as promising candidates for biomedical applications targeting oxidative stress. Thus, we show that Syzygium cumini–mediated green synthesis can produce structurally stable CaO nanoparticles with significant antioxidant activity, thereby contributing to the development of eco-friendly nanomaterials for oxidative stress–related biomedical applications.
Keywords
Syzygium cumini; green synthesis calcium oxide nanoparticles; nanobiotechnology, antioxidant activity; free radical scavenging
Citation
Srinivasan et al. Bioinformation 22(6): 3338-3342(2026)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
