Title
Multi-epitope design against emerging nipah virus towards peptide vaccine development
Authors
Motukuri Naveen Kumar1, Dokka Muni Kumar1,*, B.V.N.V. Bharathi1, Vamseedhar Annam2, Aarthi Pradhan3, Shaik Rajiya Sulthana1, Yashasvi Singam1
Affiliation
1Department of Life Sciences, School of Allied and Healthcare Sciences, Malla Reddy University, Hyderabad, Telangana, India; 2Department of Pathology, Sapthagiri Institute of Medical Sciences and Research Center, Bangalore, India; 3Department of Microbiology, Naran Lala College of Professional and Applied Sciences, Veer Narmad South Gujarat University, Surat, Gujarat, India; *Corresponding author
Motukuri Naveen Kumar - E-mail: 2211bs110039@mallareddyuniversity.ac.in
Dokka Muni Kumar - Email: drmunikumar@mallareddyuniversity.ac.in
B.V.N.V. Bharathi - E-mail: bharathi.b@mallareddyuniversity.ac.in
Vamseedhar Annam - E-mail: drvamseedharpathology@simsrc.edu.in
Aarthi Pradhan - E-mail: aarthipradhan@naranlalacollege.edu.in
Shaik Rajiya Sulthana - E-mail: 2211bs110048@mallareddyuniversity.ac.in
Yashasvi Singam - E-mail: 2211bs110093@mallareddyuniversity.ac.in
Article Type
Research Article
Date
Received June 1, 2026; Revised June 30, 2026; Accepted June 30, 2026, Published June 30, 2026
Abstract
The lack of an approved vaccine against Nipah virus (NiV), a highly fatal zoonotic pathogen causing severe neurological and respiratory disease, remains a major global health challenge. Therefore, it is of interest to design a multi-epitope vaccine targeting the NiV phosphoprotein (UniProt ID: Q9IK91). Conserved B-cell, cytotoxic T-lymphocyte (CTL) and helper T-lymphocyte (HTL) epitopes were identified and selected based on antigenicity, immunogenicity and safety. The resulting vaccine construct was evaluated through structural modeling, TLR4 docking, codon optimization and immune simulation analyses. Thus, data shows the favorable stability, strong receptor interaction, efficient expression potential and the ability to elicit robust humoral and cellular immune responses.
Keywords
Nipah virus (NiV), multi-epitope vaccine, immunoinformatics; reverse vaccinology; phosphoprotein, in silico analysis; immune simulation
Citation
Kumar et al. Bioinformation 22(6): 3786-3798 (2026)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
