Title
Comparison of MTAD and Q-mix role in growth factor mobilization, quantification and drug molecule scaffold in regenerative endodontics
Authors
Vibha Hegde1, Shilpa Shetty2,*, Rishikesh K Meshram3 & K Nandan Rao2
Affiliation
1Department of Conservative dentistry and Endodontics, Dr. G.D Pol Foundation, YMT Dental College and Hospital, Khargar, Navi Mumbai, India; 2Department of Conservative dentistry and Endodontics, Tatyasaheb Kore Dental College and Research Centre, Kolhapur, Maharashtra, India; 3Department of Conservative dentistry and Endodontics, Swargiya Dadasaheb Kalmegh Smruti Dental College and Hospital, Wanadongri Hingna, Nagpur, Maharashtra, India; *Corresponding author
Vibha Hegde - E-mail: vibhahegde26@gmail.com; Phone: +91 7400465066
Shilpa Shetty - E-mail: drshilpashetty7@gmail.com; Phone: +91 9503694873
Rishikesh K Meshram - E-mail: dr.rishikm@gmail.com; Phone: +91 7038997875
K Nandan Rao - E-mail: nandy_rao@yahoo.co.in; Phone: +91 7798691196
Article Type
Research Article
Date
Received July 1, 2026; Revised July 31, 2026; Accepted July 31, 2026, Published July 31, 2026
Abstract
Regenerative endodontics is based on the three factors of stem cells, scaffolds and growth factors where dentin matrix acts as a source of bioactive molecules. The mechanism by which irrigants can release these growth factors out of dentin without damaging the scaffold structures is an insufficiently investigated mechanism. Therefore, it is of interest to assess the effectiveness of both the mixtures of tetracycline isomer, acid and detergent (MTAD) and Tetraclean in releasing growth factors within root canal dentin and how these factors impacted triple antibiotic paste scaffolds. Sixty human premolars extracted were incubated with MTAD, Tetraclean or saline and the release of growth factor (TGF- 1, VEGF, BMP-2) measured by ELISA. MTAD showed a greater mobilization of growth factors (p<0.001) and superior stability of the scaffold than that of Tetraclean. Thus, data shows the both experimental irrigants increased the levels of growth factors as compared to the controls and these findings support their role in regenerative endodontic procedures.
Keywords
Mixtures of tetracycline isomer, acid and detergent (MTAD), Tetraclean, growth factor, regenerative endodontics, dentin matrix, scaffold.
Citation
Hedge et al. Bioinformation 22(7): 3799-3803 (2026)
Edited by
Rashmi Laddha
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
