Title
Randomized controlled clinico-pathological study of ethanol toxicity in rats and its amelioration with commercially available herbal preparations
Authors
A.N.Prakash1,*, Biswajit Dutta1, Mridusmrita Buragohain1, Parsha J. Nath2, Mousumi Hazarika3 & Nikhithasree Pullagura4
Affiliation
1Department of Veterinary Pathology, College of Veterinary Science, Guwahati, Assam – 781022, India; 2Department of Veterinary Surgery and radiology, College of Veterinary Science, Guwahati, Assam – 781022, India; 3Department of Veterinary Biochemistry, College of Veterinary science, Guwahati, Assam – 781022, India; 4Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Kerala, India; *Corresponding author
A.N. Prakash - E-mail: prakashvet6713@gmail.com
Biswajit Dutta- E-mail: drbiswajitkvk@gmail.com
Mridusmrita Buragohain- E-mail: mridusmritaburagohain@gmail.com
Parsha J. Nath- E-mail: parsha.J.Nath@aau.ac.in
Mousumi Hazarika- E-mail: mousumihazarika5@gmail.com
Nikhithasree Pullagura- E-mail: nikhithasree44@gmail.com
Article Type
Research Article
Date
Received July 1, 2026; Revised July 31,
2026; Accepted July 31, 2026, Published July 31, 2026
Abstract
Chronic alcohol consumption causes oxidative stress, liver damage and multi-organ toxicity. Therefore, it is of interest to evaluate the hepatoprotective and renoprotective effects of Liv-52, Rohitakarista, their combination and Silymarin against ethanol-induced toxicity in rats. Thirty-six healthy adult rats were divided into six groups and administered ethanol followed by the respective treatments, after which serum biochemical markers, hepatic MDA levels, triglycerides, bilirubin, creatinine, organ weights, body weight gain and histopathology of liver and kidney tissues were assessed. Ethanol significantly elevated GGT, AST, ALT, triglycerides, bilirubin, creatinine, hepatic MDA and organ weights while reducing body weight gain (p ≤ 0.05). Liv-52 significantly improved liver function markers and reduced hepatic MDA, Rohitakarista more effectively lowered creatinine and kidney weight and both Silymarin and the Liv-52 + Rohitakarista combination produced the most comprehensive protection with near-normal biochemical and histological profiles. Thus, Liv-52 and Rohitakarista demonstrated marked hepatorenal protective effects and their combination exhibited synergistic benefits comparable to or exceeding those of Silymarin.
Keywords
Ethanol-induced toxicity; Liv-52; rohitakarista; silymarin; hepatoprotection; oxidative stress; serum biochemical markers; antioxidant activity; histopathology; experimental rat model
Citation
Prakash et al. Bioinformation 22(7): 3849-3853 (2026)
Edited by
Rashmi Laddha
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
