Title

Different storage media affect shear bond strength and ARI scores in orthodontic brackets: An in vitro study

Authors

Anjali Sanjay Kamble*, Chetan Patil, Omkar A. Chinke, Rucha Jadhav, Vedant Dilip Godkar & Prajwal Rane

Affiliation

Department of Orthodontics and Dentofacial Orthopaedics, Yogita Dental College and Hospital, Khed, Maharashtra, India; *Corresponding author

Email

Anjali Sanjay Kamble - E-mail: kambleanjalisanjay@gmail.com

Chetan Patil - E-mail: drchetan1@yahoo.co.in

Omkar A. Chinke - E-mail: omkarchinke1@gmail.com

Rucha Jadhav - E-mail: rucha123@gmail.com

Vedant Dilip Godkar - E-mail: vedantgodkar8@gmail.com

Prajwal Rane - E-mail: prajwalrane20@gmail.com

Article Type

Research Article

Date

Received July 1, 2026; Revised July 31, 2026; Accepted July 31, 2026, Published July 31, 2026

Abstract

Shear bond strength (SBS) of orthodontic brackets may be influenced by the storage media used for extracted teeth in in vitro bonding studies. Hence, 120 extracted premolars were allocated to six storage media for 30 days before bracket bonding, followed by SBS testing with a universal testing machine and Adhesive Remnant Index (ARI) assessment. SBS differed significantly among the groups (p<0.001), with artificial saliva (13.92±2.69 MPa) and saline (13.38±2.51 MPa) producing the highest values, whereas ethanol (8.24±2.19 MPa) and formalin (9.67±2.44 MPa) showed the lowest. ARI score distribution did not differ significantly between the storage media (p=0.214), indicating comparable adhesive failure patterns despite differences in bond strength. Thus, data shows that physiologic storage media, particularly artificial saliva and saline, provided more consistent and clinically relevant SBS values than dehydrating or tissue-fixative media for orthodontic bonding research.

Keywords

Orthodontic brackets; shear bond strength (SBS); adhesive remnant index; storage media; artificial saliva; enamel bonding; in vitro study

Citation

Kamble et al. Bioinformation 22(7): 4176-4181 (2026)

Edited by

Hiroj Bagde

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.