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Title

Root dentin microcrack formation using hand and rotary files: An in vitro comparative study

 

Authors

Akanksha Kumari*, Ajay Kumar Nagpal, Anupama Pradhan, Arunima Jana, Seemran Panda & Astha Bhargava

 

Affiliation

Department of Conservative Dentistry and Endodontics, K.D. Dental College and Hospital, Mathura, Uttar Pradesh, India; *Corresponding author

 

Email

Akanksha Kumari - E-mail: akki212022@gmail.com

Ajay Kumar Nagpal - E-mail: drajayendodontist@gmail.com

Anupama Pradhan - E-mail: pradhananu37@gmail.com

Arunima Jana - E-mail: arunimajana1998@gmail.com

Seemran Panda - E-mail: seemranpanda4@gmail.com

Astha Bhargava - E-mail: asthabhargava03@gmail.com

 

Article Type

Research Article

 

Date

Received July 1, 2026; Revised July 31, 2026; Accepted July 31, 2026, Published July 31, 2026

 

Abstract

Dentinal microcracks during root canal instrumentation pose a serious risk by weakening root structure and predisposing teeth to vertical root fractures. Therefore, it is of interest to compare the incidence of dentinal microcracks produced by HyFlex CM rotary file system, HanuDent Blue rotary file system, NT Rainbow S rotary file system and conventional hand K-files in extracted premolars under standardized conditions. Fifty specimens were prepared, sectioned at 2, 4 and 6 mm and evaluated under a stereomicroscope with defects scored and analyzed using the chi-square test (p ≤ 0.05). Results showed that hand K-files produced the least dentinal damage, while rotary systems especially HyFlex CM and HanuDent Blue generated significantly more microcracks, with NT Rainbow S performing comparatively better among rotary instruments. Thus, data show that file design and metallurgy significantly influence dentinal preservation, supporting the selection of safer instrumentation systems in endodontic practice.

 

Keywords

Dentinal microcracks, biomechanical preparation, rotary file systems, hand k-files root dentine integrity

 

Citation

Kumari et al. Bioinformation 22(7): 4370-4374 (2026)

 

Edited by

Ritik Kashwani

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.