Title
HPLC-based hemoglobinopathy diagnosis in clinically suspected North Indian tertiary-care patients
Authors
Zoya Hussain*, Kanak Atri, Seema Goel & Janhvi Srivastav
Affiliation
Department of Pathology, School of Medical Sciences and Research (SMS&R), Sharda University, Greater Noida, Uttar Pradesh, India; *Corresponding author
Zoya Hussain -E-mail: zoyahussain.in@gmail.com
Seema Goel1 -E-mail: seema.goel@sharda.ac.in
Zoya Hussain -E-mail: zoyahussain.in@gmail.com
Seema Goel1 -E-mail: seema.goel@sharda.ac.in
Article Type
Research Article
Date
Received July 1, 2026; Revised July 31, 2026; Accepted July 31, 2026, Published July 31, 2026
Abstract
Hemoglobinopathies pose a major diagnostic burden in India, where β-thalassemia trait affects 3–15% of the population regionally and most carriers go undetected until symptomatic. Therefore, it is of interest to evaluate the 608 clinically suspected patients at Sharda Hospital, Greater Noida over 44 months using complete blood count, peripheral smear and HPLC (Bio-Rad Variant II). Abnormal hemoglobin patterns were found in 48 patients (7.89%), most commonly β-thalassemia trait (50%), followed by raised HbF and sickle cell trait (10.4% each), HbD-Punjab trait (6.2%) and rare compound heterozygous variants. MCV below 82 fL and MCH below 27 pg identified 87.5% and 95.8% of β-thalassemia trait cases respectively, while HPLC alone resolved compound heterozygous states missed by electrophoresis and solubility testing. Thus, HPLC combined with red cell indices strengthens carrier detection and supports targeted antenatal and premarital referral where nutritional anemia may confound interpretation.
Keywords
Hemoglobin (Hb), high-performance liquid chromatography (HPLC), red cell indices; hemoglobinopathies, thalassemia, sickle cell anemia, primary care
Citation
Hussain et al. Bioinformation 22(7): 4408-4411 (2026)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
