Title
Effect of EDTA demineralization duration on cryogenically nanogrinded dentin matrix: A pilot study
Authors
Shaikh Wasifoddin A. Chaudhariš,*, Sameer K. Jadhav2, Manu Bansal1, S. Ragunandhakumar3, Kiran V. Wanve4 & Mahesh R. Shrimangale4
Affiliation
1Department of Conservative Dentistry and Endodontics, Jaipur Dental college, Jaipur-302028, Rajasthan, India; 2Department of Conservative Dentistry and Endodontics, Symbiosis Centre for Health Care (SCHC) & Symbiosis University Hospital and Research Centre (SUHRC), Symbiosis International (Deemed University), Pune, Maharashtra, India.; 3Department of Pharmacology, Saveetha Dental College and Hospitals, Chennai-600077, Tamil Nadu, India 4Department of Conservative Dentistry and Endodontics, Saraswati Dhanwantari Dental College and Hospital, Parbhani-431402, Maharashtra, India; *Corresponding author
Shaikh Wasifoddin A. Chaudhari - E-mail: wasifoddin.chaudhari@yahoo.in
Sameer K. Jadhav - E-mail: dr.jadhavsameer@gmail.com
Manu Bansal - E-mail: bansaldrmanu@gmail.com
S. Ragunandhakumar - E-mail: ragunandhakumar@gmail.com
Kiran Wanve - E-mail: k.wanve92@gmail.com
Mahesh Shrimangale - E-mail: maheshshrimangale605@gmail.com
Article Type
Research Article
Date
Received July 1, 2026; Revised July 31, 2026; Accepted July 31, 2026, Published July 31, 2026
Abstract
The lack of standardized demineralization protocols limits the bioactivity of dentin-derived biomaterials. Therefore, it is of interest to evaluate the effect of different durations of 12% EDTA demineralization on cryogenically nanogrinded dentin matrix (nDDM). Nanogrinded dentin samples were treated with 12% EDTA for 0, 2, 5, 10, 30 and 60 minutes and analyzed using Fourier Transform Infrared (FTIR) spectroscopy, while biological performance was assessed by ELISA, MTT assay and qRT-PCR in human dental pulp stem cells (hDPSCs). FTIR showed preservation of non-collagen associated amide bands and the 5-minute group exhibited the highest cytocompatibility, increased BMP-2 and TGF-β1 release and enhanced expression of DMP-1 and DSPP. Prolonged demineralization beyond 5 minutes resulted in reduced biological performance and alterations in matrix-associated spectral characteristics, indicating excessive demineralization. Thus, data shows that 12% EDTA demineralization for 5 minutes provides an optimal balance between mineral removal and non-collagenous matrix preservation, supporting the regenerative potential for vital pulp therapy.
Keywords
Demineralized dentin matrix, Ethylenediaminetetraacetic acid (EDTA) demineralization, cryogenic nanogrinding, regenerative endodontics, fourier transform infrared spectroscopy (FTIR) spectroscopy, human dental pulp stem cells.
Citation
Chaudhari et al. Bioinformation 22(7): 4521-4527 (2026)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
