Title

Effect of triple antibiotic paste and calcium hydroxide intracanal medicaments on dentin microhardness

Authors

Mohd Zeeshan Ahmad¹, Jaskaran Singh², Pallawi Pallawi³, S.M. Pournima⁴, Neekita Kapil^5,* & N.L. Jhanvi⁶

Affiliation

¹Department of Conservative Dentistry and Endodontics, Rama Dental College Hospital & Research Centre, Kanpur, Uttar Pradesh, India; ²Department of Conservative Dentistry and Endodontics, Desh Bhagat Dental College and Hospital, Mandi, Gobindgarh, Punjab, India; ³Department of Dentistry, Medical College, Nalanda Medical College & Hospital, Patna, Bihar, India; ⁴Department of Dentistry, Sri Venkateshwaraa Dental college, Ariyur, Puducherry, India; ⁵Department of Pediatric and Preventive Dentistry, Guru Nanak Dev Dental College and Research Institute, Sunam, Punjab, India; ⁶Department of Dentistry, Malla Reddy Institute of Dental Sciences, Hyderabad, India; *Corresponding author

Email

Mohd Zeeshan Ahmad - E-mail: askzeeshan9@gmail.com
Jaskaran Singh - E-mail: jaskaransinghendo@gmail.com
Pallawi Pallawi- E-mail: pallawi13dec@gmail.com
S.M. Pournima - E-mail: drpournima@svdcpondy.ac.in
Neekita Kapil - E-mail: neekita.kapil@gmail.com
N.L. Jhanvi – E-mail: nanubalajhanvi@gmail.com

Article Type

Ritik Kashwani

Date

Received August 1, 2026; Revised August 31, 2026; Accepted August 31, 2026, Published August 31, 2026

Abstract

Intracanal medicaments may alter dentin microhardness, potentially compromising the structural integrity of endodontically treated teeth. Therefore, it is of interest to compare the effects of triple antibiotic paste (TAP), calcium hydroxide (Ca(OH)₂) and a control on root dentin microhardness using extracted human teeth and a Vickers hardness tester. Both medicaments significantly reduced dentin microhardness compared with the control at the coronal, middle and apical thirds (p < 0.05). TAP produced the greatest reduction in microhardness, whereas calcium hydroxide better preserved dentin hardness, indicating its superior suitability as an intracanal medicament. Thus, data show the preferential use of calcium hydroxide we encourage further research on natural intracanal medicaments and advanced methods for assessing dentin integrity.

Keywords

Calcium hydroxide (Ca(OH)₂), triple antibiotic paste (TAP), microhardness, intra-canal medicament, root canal treatment, vickers hardness.

Citation

Ahmad et al. Bioinformation 22(8): 4554-4557 (2026)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.