Title

Evaluation of antimicrobial efficacy: Herbal irrigants versus conventional irrigants

Authors

Sanjula Sirohi^1,*, Sarita Gill², Smita Durga Dutta³, Devna Sharma⁴, Roopam Kapoor⁵ & Varun Pal⁶

Affiliation

¹Department of Conservative Dentistry and Endodontics, Kalka Dental College, Meerut, Uttar Pradesh, India; ²Department of Conservative Dentistry and Endodontics, Center for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, India; ³Department of Conservative Dental Sciences, College of Dentistry, Qassim University, Saudi Arabia; ⁴Department of Conservative Dentistry and Endodontics, Faculty of Dental Sciences, SGT University, Budhera Village, Gurugram, Haryana, India; ⁵Department of Pediatric and Preventive Dentistry, Maharishi Markandeshwar College of Dental Sciences and Research, Mullana (Ambala), Haryana, India; ⁶Department of Conservative Dentistry and Endodontics, Teerthanker Mahaveer Dental College and Research Centre, TMU, Moradabad, Uttar Pradesh, India; *Corresponding author

Email

Sanjula Sirohi - E-mail: sanjulasirohi@gmail.com; Phone: +91 8506050124
Sarita Gill - E-mail: gillsarita36@gmail.com
Smita Durga Dutta - E-mail: s.dutta@qu.edu.sa
Devna Sharma - E-mail: drdevna_fds@sgtuniversity.org
Roopam Kapoor - E-mail: roopamkapoor90@gmail.com; Phone: +91 9991292168
Varun Pal - E-mail: drvarunpal@gmail.com; Phone: +91 9410095008

Article Type

Research Article

Date

Received August 1, 2026; Revised August 31, 2026; Accepted August 31, 2026, Published August 31, 2026

Abstract

Conventional endodontic irrigants like sodium hypochlorite and chlorhexidine provide strong antibacterial action but carry cytotoxicity, allergic reactions and periapical tissue damage risks requiring biocompatible alternatives. Therefore, it is of interest to compare the antibacterial efficacy of herbal irrigants (neem, triphala, green tea, aloe vera) versus conventional agents against Enterococcus faecalis using standardized microbiological protocols across 80 specimens. Colony-forming unit (CFU) assays will quantify microbial reduction in each treatment group following appropriate randomization and incubation procedures. Herbal extracts show promising antibacterial and antibiofilm properties against common endodontic pathogens in preliminary studies. Thus, data shows herbal irrigants as safer, biocompatible alternatives to chemical disinfectants for comprehensive root canal system decontamination.

Keywords

Herbal irrigants, sodium hypochlorite (NaOCl), chlorhexidine (CHX), antimicrobial efficacy, endodontics, Enterococcus faecalis

Citation

Sirohi et al. Bioinformation 22(8): 4887-4891 (2026)

Edited by

Vini Mehta

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.