Title

Diagnostic and prognostic utility of high fluorescent lymphocyte count (HFLC) and systemic immuneinflammatory index (SII) in dengue infection using sysmex XN-1000 analyzer

Authors

Gyanendra Singh¹, Tarang Patel¹, Payal Bhatt¹, Kesha Rachani^1,*, Abhishek Padhi², Ashwini Agarwal², Vishal Tayade³ & Sourabh Dwivedi¹

Affiliation

¹Department of Pathology, All India Institute of Medical Sciences (AIIMS), Rajkot, Gujarat, India; ²Department of Microbiology, All India Institute of Medical Sciences (AIIMS), Rajkot, Gujarat, India; ³Department of Pathology, Grant Medical College and Sir J.J. Group of Hospitals, Mumbai, Maharashtra, India; *Corresponding author

Email

Gyanendra Singh - E-mail: gyanendra002@gmail.com
Tarang Patel - E-mail: tarangpatel_86@yahoo.co.in
Payal Bhatt - E-mail: pbhatt1993@gmail.com
Kesha Rachani - E-mail: drkesharachani@gmail.com
Abhishek Padhi - E-mail: abhi038450@gmail.com
Ashwini Agarwal - E-mail: ash.afmc@gmail.com
Vishal Tayade - E-mail: vishaltayade07@gmail.com
Sourabh Dwivedi - E-mail: sourabhdwivedid@gmail.com

Article Type

Research Article

Date

Received August 1, 2026; Revised August 31, 2026; Accepted August 31, 2026, Published August 31, 2026

Abstract

Early diagnosis and prognostic assessment of dengue remain challenging in resource-limited settings because conventional serological tests are often costly, time-consuming and not readily available. High Fluorescence Lymphocyte Cell percentage (HFLC%) has emerged as a potential hematological marker for dengue diagnosis, while the utility of the Systemic Immune-Inflammation Index (SII) remains unclear. Hence, 118 serologically confirmed dengue cases and 50 healthy controls were evaluated using hematological parameters obtained from the Sysmex XN-1000 analyzer. Dengue patients showed higher HFLC% with good diagnostic accuracy of 82.2% sensitivity and 72.0% specificity. Thus, data shows that HFLC% is a rapid, cost-effective and reliable parameter for early dengue detection, whereas SII has limited diagnostic and prognostic value.

Keywords

Dengue fever, high fluorescent lymphocyte count (HFLC), systemic immune-inflammatory index (SII)

Citation

Singh et al. Bioinformation 22(8): 4975-4980 (2026)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.