Title
Efficiency evaluation of different activation methods on dentinal tubule penetration by a bioactive glass-based sealer using confocal laser scanning microscopy: An in vitro study
Authors
Rupali Priyadarshi, Sanjeev Srivastava*, Arohan Singh, Saraswati Sachan & Prachi Jha
Affiliation
Department of Conservative Dentistry and Endodontics, Sardar Patel Post Graduate Institute of Dental and Medical Sciences, Lucknow, Uttar Pradesh, India; *Corresponding author
Rupali Priyadarshi - E-mail:
priyadarshi.rupali@gmail.com
Sanjeev Srivastava - E-mail: sanjeevsrivastava409@gmail.com
Arohan Singh - E-mail: arohansingh@gmail.com
Saraswati Sachan - E-mail: saraswatisachan90@gmail.com
Prachi Jha - E-mail: prachijha1996@gmail.com
Article Type
Research Article
Date
Received August 1, 2026; Revised August 31, 2026; Accepted August 31, 2026, Published August 31, 2026
Abstract
Dentinal tubule penetration of bioactive glass-based sealers is influenced by the activation technique used during obturation. Therefore, it is of interest to compare the ultrasonic, sonic, lentulospiral and manual activation methods for dentinal tubule penetration of a bioactive glass-based sealer using confocal laser scanning microscopy. Sixty extracted mandibular premolars were prepared, obturated after sealer activation (n=15/group) and evaluated at coronal, middle and apical thirds using CLSM. Ultrasonic activation produced the greatest penetration at all root levels (322.58±4.34, 295.74±4.37 and 197.42±4.22 μm), followed by sonic (291.02 μm ,250.55 μm and 179.69 μm), lentulospiral (265.75 μm, 213.77 μm and 145.21 μm) and manual (155.47 μm, 114.03 μm and 95.70 μm) activation, with significant intergroup differences (P<0.001). Thus, data shows that ultrasonic activation significantly enhances dentinal tubule penetration of bioactive glass-based sealer and may improve obturation quality compared with conventional techniques.
Keywords
Bioactive glass–based sealer; dentinal tubule penetration; ultrasonic activation; sonic activation; lentulospiral; confocal laser scanning microscopy (CLSM)
Citation
Priyadarshi et al. Bioinformation 22(8): 5109-5114 (2026)
Edited by
Vini Mehta
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
