Title
Evaluation of bovine pericardial regenerative membrane in the presence and absence S9 activation using Ames test
Authors
Akram Hassan1, Abdo Mohammed Mohammed Abdulrazzaq2,*, Mohammed Mohsen Al Jearah2 , Ali Saleh Alqahtani3, Muhammad Junaid4 & Maryam Junaid5
Affiliation
1Department Of Dental Sciences, Health Campus, Universiti Sains Malaysia (USM), Kelantan, Malaysia; 2Department of Preventive Dental Science, Faculty of Dentistry, Najran University, Najran, Saudi Arabia; 3Department of Prosthetic Dental Science, Faculty of Dentistry, Najran University, Najran, Saudi Arabia; 4Department of Endodontist, Najran Health Cluster, Ministry of Health, Najran, Saudi Arabia; 5Department of Pediatric Dentistry, University of Greifswald, Germany; *Corresponding author
Akram Hassan - E-mail: akram@usm.my;
Phone: +6012-9601791
Abdo Mohammed Mohammed Abdulrazzaq - E-mail:
dr.alra2ki2004@gmail.com; Phone: +966 561855610
Mohammed Mohsen Al Jearah - E-mail: mmaljerah@nu.edu.sa; Phone:
00966506794076
Ali Saleh Alqahtani - E-mail: asalqhtani@nu.edu.sa; Phone: +966
503059500
Muhammad Junaid - E-mail: dr.jkhan24@gmail.com; mujumuhammad@moh.gov.sa
Maryam Junaid - E-mail: brazencharriot@yahoo.com; openaccess@uni-greifswald.de
Article Type
Views
Date
Received August 1, 2026; Revised August 31, 2026; Accepted August 31, 2026, Published August 31, 2026
Abstract
Bovine pericardial membranes are widely used in guided tissue regeneration (GTR). However, their genetic safety has not been fully established. Therefore, it is of interest to evaluate the genotoxicity of bovine pericardial as a GTR material in the presence and absence of S9 activation in Salmonella typhimurium strains TA98, TA100, TA1535 and TA1537, at concentrations of 0.3125–5 mg/plate using the Ames test. We found no biologically relevant increase in revertant colonies. Thus, bovine pericardial is non-mutagenic and supports its preclinical genetic safety for periodontal regeneration applications. Further in vivo studies are warranted to confirm its long-term safety.
Keywords
Genotoxicity, mutation assay, bovine pericardial, guided tissue regeneration (GTR)
Citation
Hassan et al. Bioinformation 22(8): 5190-5194 (2026)
Edited by
P Kangueane
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
