Title

CeraSeal bioceramic sealer dentinal tubule penetration after three irrigant activation methods: Laboratory study

Authors

Mahira Palottukandy*, Rekha P. Thankachan, Anoop Samuel, Reshma Rajasekhar, K.N. Aiswarya & Ishra M. Koya

Affiliation

Department of Conservative Dentistry and Endodontics, MES Dental College, Perinthalmanna, Kerala, India; *Corresponding author

Email

Mahira Palottukandy - E-mail: pkmahira1993@gmail.com
Rekha P. Thankachan - E-mail: rekhasamuel7@gmail.com
Anoop Samuel - E-mail: anoopzsam@gmail.com
Reshma Rajasekhar - E-mail: reshmarajasekhar@gmail.com
K.N. Aiswarya - E-mail: aiswaryakn1234@gmail.com
Ishra M. Koya - E-mail: ishrakoya9@gmail.com

Article Type

Research Article

Date

Received August 1, 2026; Revised August 31, 2026; Accepted August 31, 2026, Published August 31, 2026

Abstract

Smear-layer persistence may restrict dentinal tubule penetration of bioceramic sealers and compromise obturation. Eighty-four mandibular premolars received three irrigant activation methods with 17% EDTA or saline. CLSM measured CeraSeal penetration, while SEM assessed smear-layer removal. EDTA increased penetration significantly, with EDTA plus PUI showing the greatest depth (1888.09 ± 67.72 μm; p < 0.001). Thus, EDTA-assisted PUI may improve CeraSeal penetration, although the SEM dataset requires verification.

Keywords

CeraSeal, bioceramic sealer, confocal laser scanning microscopy, dentinal tubule penetration, manual dynamic agitation, passive ultrasonic irrigation, smear layer

Citation

Palottukandy et al. Bioinformation 22(8): 5309-5313 (2026)

Edited by

Hiroj Bagde

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.