Title
Comparative evaluation of ProRoot MTA, MTA Angelus and BioStructure MTA Putty for retrograde filling using dye penetration with stereomicroscopic analysis: An in vitro study
Authors
A.B. Harishankar*, Anoop Samuel, Rekha P. Thankachan, Emil Santhosh Mani, S. Hridya & K. Anusha
Affiliation
Department of Conservative Dentistry and Endodontics, MES Dental College, Perinthalmanna, Kerala, India; *Corresponding author
A.B. Harishankar - E-mail: harisankarab@gmail.com
Anoop Samuel - E-mail: anoopzsam@gmail.com
Rekha P. Thankachan - E-mail: rekhasamuel7@gmail.com
Emil Santhosh Mani - E-mail: dremilsmani@gmail.com
S. Hridya - E-mail: hridyas654@gmail.com
K. Anusha - E-mail: anusharajk2017@gmail.com
Article Type
Research Article
Date
Received August 1, 2026; Revised August 31, 2026; Accepted August 31, 2026, Published August 31, 2026
Abstract
Root-end filling materials must provide an effective apical seal to minimize microleakage and support periapical healing. Therefore, it is of interest to compare the sealing ability of ProRoot MTA, MTA Angelus and Safe Endo Biostructure MTA Putty as retrograde filling materials using dye penetration and stereomicroscopic analysis. Thirty extracted human mandibular premolars were instrumented, obturated, subjected to root-end resection and ultrasonic retrograde cavity preparation and randomly allocated to three groups (n = 10) according to the retrograde filling material used, followed by immersion in 2% methylene blue dye and evaluation of linear dye penetration under a stereomicroscope. Intergroup comparisons were performed using one-way ANOVA followed by Tukey's post-hoc test. ProRoot MTA showed significantly lower dye penetration than MTA Angelus and Safe Endo Biostructure MTA Putty (p < 0.001), whereas no statistically significant difference was observed between MTA Angelus and Safe Endo Biostructure MTA Putty (p = 0.799). Thus, data shows that ProRoot MTA showed the best apical sealing ability among the materials evaluated and may be considered a more effective retrograde filling material for minimizing microleakage.
Keywords
ProRoot MTA, MTA angelus, bio structure MTA putty, stereomicroscope
Citation
Harishankar et al. Bioinformation 22(8): 5361-5366 (2026)
Edited by
Hiroj Bagde
ISSN
0973-2063
Publisher
License
This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.
