Title

Glucose and lipid storage stability: Effects on insulin-resistance indices

Authors

Saket Verma¹, Sushma Kumari², Abhay Kumar³, Usha Saroj⁴, Abhiraj Ramchandani⁵, Kirti Ramchandani⁶, Priyanka Shrivastava⁷,*, Amlan Swain⁸ & Seelora Sahu⁸

Affiliation

¹Department of Biochemistry (Trauma Centre), Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India; ²Department of Transfusion Medicine and Blood Centre, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India;³Department of Medicine, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India; ⁴Department of Pathology, Shahid Nirmal Mahto Medical College, Dhanbad, Jharkhand, India; ⁵Department of Pathology, Amaltas Institute of Medical Sciences, Dewas, India;⁶Department of Microbiology, R. D. Gardi Medical College, Ujjain, Madhya Pradesh, India; ⁷Department of Anesthesia, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India; ⁸Department of Anesthesia, Tata Main Hospital, Jamshedpur, Jharkhand, India; *Corresponding author

Email

Saket Verma - E-mail: drsaketverma@gmail.com
Sushma Kumari - E-mail: ksushmaxyz@rediffmail.com
Abhay Kumar - E-mail: drabhaykumarran@gmail.com
Usha Saroj - E-mail: ushasaroj1707@gmail.com
Abhiraj Ramchandani - E-mail: abhirajramchandani@gmail.com
Kirti Ramchandani - E-mail: kirtihemwani@rediffmail.com
Priyanka Shrivastava - E-mail: shrivastava2911@gmail.com
Amlan Swain - E-mail: amlanswain1@gmail.com
Seelora Sahu - E-mail: seelora@gmail.com

Article Type

Research Article

Date

Received September 1, 2026; Revised September 30, 2026; Accepted September 30, 2026, Published September 30, 2026

Abstract

Delayed processing and inappropriate storage can alter glucose and lipid measurements and may introduce clinically relevant error into derived insulin-resistance indices. Therefore, it is of interest to evaluate the glucose, triglycerides and HDL cholesterol in aliquots from 100 fasting specimens under room-temperature storage, refrigeration, freezing and repeated freeze-thaw conditions and assessed the propagated effects on the TyG index and TG/HDL-C ratio. At room temperature, glucose declined by 1.8%, 3.9%, 7.8% and 18.6% at 2, 4, 8 and 24 hours, respectively, while triglycerides remained within 3% of baseline and HDL-C declined by 4.1% at 24 hours. TyG bias reached -2.9% at 24 hours with 17% reclassification; refrigeration preserved all analytes for 24 hours with minimal index reclassification, 30-day frozen storage produced small biases and three freeze-thaw cycles increased TG/HDL-C by 11.0%. Thus, delayed room-temperature separation predominantly affected glucose and could underestimate TyG, whereas prompt separation with refrigeration preserved derived indices for at least 24 hours and repeated freeze-thaw cycles disproportionately altered TG/HDL-C.

Keywords

Pre-analytical variation, glucose stability, triglycerides, HDL cholesterol, TyG index, TG/HDL ratio, storage temperature, freeze-thaw

Citation

Verma et al. Bioinformation 22(9): 5589-5593 (2026)

Edited by

Ashwini Dhopte

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.