Title

Non-invasive biomarker-based evaluation of periapical bone healing using salivary and urinary deoxypyridinoline: A prospective cohort study

Authors

Priyanka Yadav¹, Ajay Chhabra¹,*, R. Shilpa¹, Arpita Banerjee¹, Sona Parvathy¹, Himani Mehra¹ & Vandana Chhabra²

Affiliation

¹Department of Dentistry, All India Institute of Medical Sciences, Kalyani, West Bengal, India; ²Department of Oral and Maxillofacial Surgery, H. S. Judge Institute of Dental Sciences and Hospital, Panjab University, Chandigarh, India; *Corresponding author

Email

Priyanka Yadav - E-mail: dentpriyanka92@gmail.com
Ajay Chhabra - E-mail: ajay.dental@aiimskalyani.edu.in
R. Shilpa - E-mail: shilpa.dental_acjr25@aiimskalyani.edu.in
Arpita Banerjee - E-mail: arpita.dental_acjr25@aiimskalyani.edu.in
Sona Parvathy - E-mail: sjp1791@gmail.com
Himani Mehra - E-mail: himani.red@gmail.com
Vandana Chhabra - E-mail: drvandanachhabra@gmail.com

Article Type

Research Article

Date

Received September 1, 2026; Revised September 30, 2026; Accepted September 30, 2026, Published September 30, 2026

Abstract

Radiographic assessment of periapical healing after non-surgical root canal treatment (NSRCT) may not reflect early biological changes and requires repeated radiation exposure, highlighting the need for reliable non-invasive biomarkers. Therefore, it is of interest to evaluate the salivary and urinary deoxypyridinoline (DPD) levels in 35 patients with periapical lesions and 40 healthy controls, correlating biomarker changes with cone-beam computed tomography (CBCT)-based healing over 3 months. Salivary and urinary DPD concentrations were measured by ELISA at baseline, 1 month and 3 months, while lesion volume was assessed volumetrically using CBCT. Both salivary and urinary DPD levels declined significantly over time (p < 0.001), showed a moderate positive correlation (r = 0.52, p = 0.001) and 97.1% of patients showed radiographic healing, although DPD changes were not significantly associated with lesion volume reduction (ρ = 0.20, p = 0.242). Thus, data shows that salivary DPD may serve as a non invasive adjunctive biomarker for monitoring periapical bone healing following NSRCT.

Keywords

Bone biomarker, cone-beam computed tomography (CBCT), deoxypyridinoline, periapical healing, root canal treatment, saliva

Citation

Yadav et al. Bioinformation 22(9): 5766-5770 (2026)

Edited by

Ritik Kashwani

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.