Title

Diagnostic efficacy of modified gram staining methods: A review

Authors

Swati Narayan Thakare & Dhruba Chandi*

Affiliation

Department of Microbiology, Jawaharlal Nehru Medical College, Datta Meghe Institute of Medical Sciences, Sawangi, Wardha, Maharashtra, India; *Corresponding author

Email

Swati Narayan Thakare - E-mail: tswati24@gmail.com
Dhruba Chandi - E-mail: dhruba.chandi@dmiher.edu.in

Article Type

Review

Date

Received September 1, 2026; Revised September 30, 2026; Accepted September 30, 2026, Published September 30, 2026

Abstract

Early diagnosis of bacterial infections is vital for prompt treatment to reduce morbidity. Conventional Gram staining provides rapid visualization. However, it suffers from low sensitivity. Bacterial culture offers definitive identification but is highly time consuming. Modified Gram stain techniques address these limitations by improving visualization in low-load specimens. However, standardized multicenter studies are required to validate these methods to establish best practices.

Keywords

Gram staining techniques, modified Gram staining, culture technique, bacterial detection, diagnostic accuracy

Citation

Narayan & Chandi, Bioinformation 22(9): 5866-5869 (2026)

Edited by

Rashmi Laddha

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.