Title

Phytochemical screening and antimicrobial activity of Syzygium cumini leaf extract: An experimental laboratory study

Authors

Renu Pathak¹,*, Harsh Pathak², Vartika Bajpai³, Vikram Karande⁴, Nazia Khan⁵, Mohd Zeeshan Ahmad⁶ & Anukriti Kumari⁷

Affiliation

¹Department of Microbiology, Rani Durgavati Vishwavidyalaya (RDVV), Jabalpur, Madhya Pradesh, India; ²Department of Medical Biotechnology, Galgotias University, Greater Noida, Uttar Pradesh, India; ³Department of Oral Medicine and Radiology, D. Y. Patil Dental School, Lohegaon, Pune, Maharashtra, India; ⁴Department of Dentistry, AIIMS, Rajkot, Gujarat, India; ⁵Department of Microbiology, Basic Medical Science, College of Medicine, Majmaah University, Al-Majmaah, Riyadh, Saudi Arabia; ⁶Department of Conservative Dentistry & Endodontics, Rama Dental College Hospital & Research Centre, Kanpur, Uttar Pradesh, India; ⁷Department of Oral Medicine and Radiology, School of Dental Sciences, Sharda University, Greater Noida, Uttar Pradesh, India; *Corresponding author

Email

Renu Pathak - E-mail: 1234pathakrenu02@gmail.com
Harsh Pathak - E-mail: harshpathak825@gmail.com
Vartika Bajpai - E-mail: drvartikavbajpai@outlook.com
Vikram Karande - E-mail: drvikramkarande@gmail.com; hoddental@aiimsrajkot.edu.in
Nazia Khan - E-mail: n.sabir@mu.edu.sa
Mohd Zeeshan Ahmad - E-mail: askzeeshan9@gmail.com
Anukriti Kumari - E-mail: anukritishrma0@gmail.com

Article Type

Research Article

Date

Received September 1, 2026; Revised September 30, 2026; Accepted September 30, 2026, Published September 30, 2026
 

Abstract

Herbal antimicrobials are increasingly being explored as safer alternatives for managing resistant microorganisms in dentistry and medicine. Therefore, it is of interest to evaluate the phytochemical composition and antioxidant and antimicrobial potential of the ethyl acetate leaf extract of Syzygium cumini using phytochemical screening, paper chromatography and agar-based antimicrobial assays. The extract contained alkaloids, flavonoids, tannins, saponins, terpenoids, proteins/amino acids and starch, while chromatography confirmed the separation of bioactive constituents. Complete inhibition of bacterial growth was observed on extract incorporated agar plates after 24 hours of incubation, showing strong antimicrobial activity. Thus, data shows that Syzygium cumini leaves as a promising natural source of bioactive compounds for developing plant-based oral healthcare products, including mouthwashes, irrigants and dentifrices.

Keywords

Syzygium cumini, medicinal plants, dentistry, irrigants, flavonoids, Jamun, antimicrobial properties, ethnomedicine, Streptococcus mutans, mouthwash, periodontal diseases

Citation

Pathak et al. Bioinformation 22(9): 6052-6056 (2026)

Edited by

Ritik Kashwani

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.