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Title

Insilico docking study of compounds elucidated from helicteres isora fruits with ampkinase- insulin receptor

 

Authors

Subramanium Vennila*, Giridharan Bupesh*, Krishnan Saravanamurali, Viajayan SenthilKumar, Ramalingam SenthilRaja, Natarajan Saran & Sachidanandam Magesh

 

Affiliation

King Institute of Preventive Medicine and Research, Guindy, Chennai-600032

 

Email

Venajay1974@gmail.com; bupeshgiri@gmail.com; *Corresponding authors

 

Article Type

Hypothesis

 

Date

Received April 05, 2014; Accepted April 17, 2014; Published May 20, 2014

 

Abstract

Insulin receptor (IR) proteins were essential intracellular signaling peptides in the insulin action cascade. Insulin receptor substrate proteins (IRS-1and IRS-2) serve and regulate the insulin level in the normal insulin action. The broad role of IRS-1 and IRS-2 in cell growth and survival reveals a common regulatory pathway linking development, somatic growth, fertility, neuronal proliferation, and aging to the core mechanisms used by vertebrates for nutrient sensing. Such type of proteins were cyclic adenosine monophosphate-activated protein kinase, this proteins play a key role in the insulin response and regulation. Type -2 Diabetes mellitus occurs during prolonged periods of peripheral insulin resistance due to inactivation of IRS proteins. The compounds isolated from the medicinal plants were safer than synthetic drugs and possess high bio activity. In the present study, four compounds were elucidated from fruits of Helicteres isora. The elucidated compounds were evaluated for the antidiabetic activity using Insilico docking study. The receptor was analyzed for the active site and pocket finder tools. The aminoacids such as Phenylalanine, Lysine, Glutamic acid and Asparigine were predicted as active site binding residues. Docking studies were done through Autodock 4 software. All the compounds from fruits of Helicteres isora showed good docking profiles with AMP Kinase, except compound-3 (1,2,3,4-tetrahydro-1,5,6,8-tetramethyl-7-(2-methylprop-1-enylnaphthalene-4-ylpivalate). Finally the result from the study demonstrates that the HS-1, HS-2 and HS-4 posses potent anti diabetic activity against type-2 diabetes mellitus through drug action on AMP kinase cascade system.

 

Keywords

Helicteres isora, Q-site finder, Autodock, Insulin receptor substrate proteins, Antidiabetic activity, Protein-drug docking.

 

Citation

Vennila et al. Bioinformation 10(5): 263-266 (2014)
 

Edited by

P Kangueane

 

ISSN

0973-2063

 

Publisher

Biomedical Informatics

 

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.