Title

Diagnostic utility of ADA and CBNAAT in tubercular pleural effusion

Authors

N Patel Ronak Kumar¹, Rahul Soni², Darshi Rastogi³, Pankaj Kumar Gupta^4,* & Sachin Parmar⁵

Affiliation

¹Department of Pulmonary Medicine, L N Medical College & J K Hospital, Bhopal, Madhya Pradesh, India; ²Department of Pulmonary Medicine, BirsaMunda Government Medical College, Shahdol, Madhya Pradesh, India; ³Department of Pulmonary Medicine, L N Medical College& J K Hospital, Bhopal, Madhya Pradesh, India; ⁴Department of General Medicine, BirsaMunda Government Medical College Shahdol, Madhya Pradesh, India; ⁵Department of Community Medicine,V.K.S. Government Medical College, Neemuch, Madhya Pradesh, India; *Corresponding author

Email

N Patel Ronak Kumar - E-mail: rnpatel6692@gmail.com
Rahul Soni - E-mail: Rssonirahul@gmail.com
Darshi Rastogi - E-mail: rrhunjun@gmail.com
Pankaj Kumar Gupta - E-mail: dr.pankajgupta89@gmail.com
Sachin Parmar - E-mail: dr.sachinparmar@gmail.com

Article Type

Research Article

Date

Received November 15, 2025; Revised December 15, 2025; Accepted December 15, 2025, Published December 15, 2025

Abstract

The diagnostic performance of Adenosine Deaminase (ADA) and Cartridge-Based Nucleic Acid Amplification Test (CBNAAT) in diagnosing tubercular pleural effusion (TPE) is of interest. Hence, a study was conducted over 18 months at LN Medical College and JK Hospital, Bhopal. The study involved 108 adult patients with TPE. Data shows that CBNAAT, although highly specific, had limited sensitivity, while ADA ≥40 IU/L demonstrated strong sensitivity. ADA levels were significantly higher in CBNAAT-positive participants, suggesting that combining both tests enhances diagnostic accuracy. This approach can help reduce the need for invasive procedures and improve timely treatment, especially in resource-limited settings. We show the importance of using ADA and CBNAAT together in the diagnosis of TPE.

Keywords

Tubercular pleural effusion, Adenosine Deaminase (ADA), CBNAAT, diagnostic accuracy, sensitivity

Citation

Kumar et al. Bioinformation 21(12): 4484-4487 (2025)

Edited by

P Kangueane

ISSN

0973-2063

Publisher

Biomedical Informatics

License

This is an Open Access article which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. This is distributed under the terms of the Creative Commons Attribution License.